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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The detection of hypoxic markers in head and neck tumours
 Šťovíčková, Eliška ; Šmahelová, Jana (advisor) ; Rösel, Daniel (referee)
Head and neck cancers (HNSCC) are heterogeneous group of tumours. Risk factors are mainly smoking and alcohol consumption. Some of these tumours are associated with human papillomavirus (HPV) infection, which is a significant positive prognostic factor. These tumours differ from HPV-negative tumours in clinicopathological characteristics, tumour microenvironment and response to treatment. Hypoxia is commonly found in tumors including HNSCC and is a significant prognostic and predictive factor. Elucidating the degree of hypoxia in relation to HPV infection could partly explain the differences in prognosis of these patients and allow more appropriate choice of therapy. Aspartate-β-hydroxylase is also a significant negative prognostic factor in a number of tumours, but its role in HNSCC has not yet been investigated. I focused on the detection of hypoxic markers and aspartate-β-hydroxylase expression in samples from HNSCC patients at the mRNA level by quantitative PCR and at the protein level by multispectral immunohistochemistry. The expression levels of these markers were compared in tumors stratified according to viral etiology and other characteristics such as smoking, localization or tumor stage. HPV infection had the highest impact on the expression of these markers, but the results cannot point to a...
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Gene expression profiling in diabetic nephropathy
 Nepomucká, Kateřina ; Pavlínková, Gabriela (advisor) ; Černá, Věra (referee)
Diabetic nephropathy (DN) is induced by both type 1 and type 2 diabetes mellitus and it is one of the most serious complications associated with diabetes. Despite increasing incidence of diabetes, the exact pathogenesis remains unclear. Hypoxia is regarded as a crucial factor for the progression of renal disease. The responses to hypoxia are mainly regulated by hypoxia-inducible factor 1 (HIF1). We thus considered a possible link between HIF1-regulated pathways and the susceptibility to DN and the disease progression. We hypothesize that the exposure of renal tissue to diabetes causes gene expression changes in HIF1-regulated pathway and the altered expression profile is decisive for the development of DN. Using mouse model, we analyzed cellular and molecular changes in HIF1 heterozygous-null (Hif1α+/- ) and wild type (wt) littermates exposed to diabetic environment. Our histological analysis showed early pathological changes associated with DN in both diabetic wt and Hif1α+/- compared to non-diabetic controls. The morphological analysis did not demonstrate the effect of Hif1α+/- genotype in comparison to wt. For our molecular analysis with qRT-PCR method, we selected several genes, which were previously associated with pathological processes in kidney diseases. We identified statistically...
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